Lupine Protein Hydrolysates Inhibit Enzymes Involved In
نویسندگان
چکیده
13 Lupine protein hydrolysates (LPHs) were obtained from a lupine protein isolate (LPI) 14 by enzymatic hydrolysis using two proteases, Izyme AL and Alcalase 2.4 L, and their 15 potential anti-inflammatory capacities were studied by determining their in vitro 16 inhibition of the following enzymes that are involved in the inflammatory process: 17 phospholipase A2 (PLA2), cyclooxygenase 2 (COX-2), thrombin, and transglutaminase 18 (TG). The strongest inhibitory activities toward PLA2 and TG were found in the 19 hydrolysates obtained by hydrolysis with Izyme and subsequently with Alcalase, with 20 more than 70% inhibition obtained in some cases. All of the hydrolysates tested 21 inhibited more than 60% of the COX-2 activity. In no case did the percentage of 22 thrombin activity inhibition exceed 40%. The best inhibitory activities were found in the 23 LPH obtained after 15 min of hydrolysis with Alcalase and in the LPH obtained after 60 24 min of hydrolysis with Izyme followed by 15 min of hydrolysis with Alcalase. Enzyme 25 *Manuscript Click here to view linked References
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